PKCα Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91080-50, BS91080-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P17252(Human) P20444(Mouse) P05696(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP：1:10-1:50
Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC and é are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PKCα polyclonal antibody detects endogenous levels of PKCα protein.
Bioworld Molecular Weight: 75 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: 293, ICC staining PKC alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA, AAG6, Aging associated gene 6, aPKC, KPCA, PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified