PKCγ Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91083-50, BS91083-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P05129(Human) P63318(Mouse) P63319(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: PKCγ polyclonal antibody detects endogenous levels of PKCγ protein.
Bioworld Molecular Weight: 78 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PKC gamma on A549 cell lysates using anti-PKC gamma antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PKC gamma antibody. Counter stained with hematoxylin.
Alternative Name: PKC-gamma, PRKCG, PKCG, KPCG, MGC57564, OTTHUMP00000067291, PKC-gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma, Protein kinase C gamma polypeptide, Protein kinase C gamma type, Protein kinase C, gamma, SCA 14, SCA14,
Immunogen: Synthetic peptide within Human PKC gamma aa 350-450.
Conjugate: Unconjugated
Modification: Unmodified