PKCδ Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS91084-50, BS91084-100 Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q05655(Human) P28867(Mouse) P09215(Rat) Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, ICC/IF, IHC All Applications: WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. Purification and Purity: ProA affinity purified Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity: PKCδ polyclonal antibody detects endogenous levels of PKCδ protein. Bioworld Molecular Weight: 78 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3, ICC staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alternative Name: PRKCD, CVID9, D14Ertd420e, Kinase PKC delta, KPCD, KPCD, MAY 1, MAY1, MGC49908, nPKC delta, nPKC-delta, PCKd, PKC d, PKC delta, PKCD, PKCdelta, PRKC D, PRKC delta, Prkcd, Protein Kinase C delta, Protein kinase C delta type, Protein kinase C delta VIII, Protein Kinase Cdelta, Tyrosine protein kinase PRKCD, Immunogen: recombinant protein Conjugate: Unconjugated Modification: Unmodified