Product Description

PKC γ (T214) Polyclonal Antibody

Catalogue Numbers: BS9195-50, BS9195-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05129

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, λ, ν, μ, ι, and θ).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PKC γ (T214) polyclonal antibody detects endogenous levels of PKC γ protein.

Extra Notes: Western blot (WB) analysis of PKC γ (T214) pAb at 1:500 dilution
Lane1: The Brain tissue lysate of Mouse(40ug)
Lane2: The Brain tissue lysate of Rat(40ug)

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein kinase C gamma type; PKC-gamma; PKCG; PRKCG

Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human PKC γ.

Conjugate: Unconjugated

Modification: Unmodified