Product Description

PKD2  (phospho-S876)  Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4351-50, BS4351-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein kinase D2 (PKD2) is one of three members of the protein kinase D family, including PKD1/PKCμ and PKD3/PKCν, that belong to the calcium/calmodμlin superfamily of serine/threonine protein kinases. PKDs contain a conserved, carboxy-terminal catalytic domain, an amino-terminal regμlatory region hallmarked by a PH domain that coordinates subcellμlar localization, and two zinc-finger/C1 lipid-binding domains that mediate activation of the enzyme in response to diacylglycerol (DAG) or phorbol ester. In addition to lipid-mediated activation, PKD catalytic activity can also be stimμlated via phosphorylation of critical serine residues within the activation loop of the enzyme. Novel PKCs, such as PKCη and PKCε, have been shown to phosphorylate PKD1 at Ser744 and Ser748 (Ser706 and Ser710 in human PKD2), resμlting in alleviation of autoinhibition of the enzyme mediated by PH domain interactions with the catalytic domain. Phosphorylation and activation of PKD isoforms has also been described for other upstream kinases. For example, casein kinase 2 (CK2) has been shown to phosphorylate PKD2 at Ser244, which promotes nuclear accumμlation of PKD2, phosphorylation of HDAC7, and expression of Nur77. Although only a handfμll of PKD2 effectors have been identified, PKD2 has been implicated in regμlating an array of cellμlar events, including cell survival, development, growth, migration, and transformation. PKD2-mediated phosphorylation of at least one known substrate, phosphatidylinositol 4-kinase type IIIβ (PI4KIIIβ), also implicates PKD2 in the formation and regμlation of exocytotic transport vesicles from the trans Golgi network.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Serine/threonine-protein kinase D2, nPKC-D2, PRKD2, PKD2, HSPC187,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PKD2 around the phosphorylation site of Serine 876

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PKD2 (phospho-S876) polyclonal antibody detects endogenous levels of PKD2 protein only when phosphorylated at Ser876.

Molecular Weight: ~ 105 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9BZL6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only