PKR Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10307-50, MB10307-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P19525
Host: Rabbit
Reactivity: Human
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 62 kDa; Observed MW: 74 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PKR in K562, Hela lysates using PKR antibody., Immunocytochemistry analysis of PKR in Hela using PKR antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using PKR antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: EIF2AK2; PKR; PRKR; Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein k
Immunogen: A synthetic peptide of human PKR
Conjugate: Unconjugated
Modification: Unmodified