Product Description

PKR Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66916-50, MB66916-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein kinase is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the 伪 subunit of the initiation factor eIF2 and also controls the activation of several transcription factors, such as NF-魏B, p53, and the Stats. In addition, PKR mediates apoptosis induced by many different stimμli, such as LPS, TNF-伪, viral infection, and serum starvation . Activation of PKR by dsRNA resμlts in PKR dimerization and autophosphorylation of Thr446 and Thr451 in the activation loop. Substitution of threonine for alanine at position 451 completely inactivated PKR, while a mutant with a threonine to alanine substitution at position 446 was partially active. Research studies have implicated PKR activation in the pathologies of neurodegenerative diseases, including Alzheimer's disease.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: PKR, PRKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, Protein kinase RNA-activated, PKR, Tyrosine-protein kinase EIF2AK2, p68 kinase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human PKR. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of PKR protein.

Molecular Weight: ~ 70 kDa

Pathway: Translational Contral Regμlation of elF2

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P19525

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only