PLA2G2D Polyclonal Antibody-BS8359

PLA2G2D Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS8359-50, BS8359-100

Swiss-Prot: Q9UNK4

Host: Rabbit

PLA2G2D Polyclonal Antibody-BS8359

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF/ICC: 1:50~1:200

Background: Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids, many of which are potent mediators of inflammation. As a member of the PLA2 family, group IID sPLA2, also known as PLA2G2D (phosphatidylcholine 2-acylhydrolase 2D), sPLA2-IID or SPLASH, is a 145 amino acid secreted protein that is broadly expressed but found at highest levels in pancreas and spleen. Group IID sPLA2 catalyzes the reaction of phosphatidylcholine and water into 1-acylglycerophosphocholine and carboxylate, and has been liked to body weight loss in patients with chronic obstructive pulmonary disease (COPD), as well as lymphotoxin deficiency.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PLA2G2D polyclonal antibody detects endogenous levels of PLA2G2D protein.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of sPLA2-IID expression in Hela (A), THP1 (B) whole cellLysates., Immunofluorescent analysis of sPLA2-IID staining in U2OS cells.

Alternative Name: Group IID secretory phospholipase A2; GIID sPLA2; sPLA2-IID; PLA2IID; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog; PLA2G2D; SPLASH

Immunogen: Synthetic peptide, corresponding to Human PLA2G2D.

Modification: Unmodified

Conjugate: Unconjugated