PLA2G3 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77414-50, BS77414-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q9NZ20 Host: Rabbit Reactivity: Human, Mouse Applications: WB All Applications: WB,1:500 - 1:2000 Background: This gene encodes a protein that belongs to the secreted phospholipase A2 family, whose members include the bee venom enzyme. The encoded enzyme functions in lipid metabolism and catalyzes the calcium-dependent hydrolysis of the sn-2 acyl bond of phospholipids to release arachidonic acid and lysophospholipids. This enzyme acts as a negative regulator of ciliogenesis, and may play a role in cancer development by stimulating tumor cell growth and angiogenesis. This gene is associated with oxidative stress, and polymorphisms in this gene are linked to risk for Alzheimer's disease. [provided by RefSeq, Apr 2014] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using PLA2G3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 57kDa Alternative Name: GIII-SPLA2; SPLA2III; sPLA2-III; PLA2G3 Immunogen: A synthetic peptide of human PLA2G3(NP_056530.2). Conjugate: Unconjugated Modification: Unmodified