Placental alkaline phosphatase (PLAP) Polyclonal Antibody-BS78166

Placental alkaline phosphatase (PLAP) Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78166-50, BS78166-100

Swiss-Prot: P05187

Host: Rabbit

Placental alkaline phosphatase (PLAP) Polyclonal Antibody-BS78166

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,50 - 1:200

Background: The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Placental alkaline phosphatase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Placental alkaline phosphatase antibody. Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase antibody at a dilution of 1:1000.

Alternative Name: ALPP; ALP; PALP; PLAP; PLAP-1; alkaline phosphatase, placental

Immunogen: Recombinant fusion protein of human Placental alkaline phosphatase (PLAP)(NP_001623.3).

Modification: Unmodified

Conjugate: Unconjugated