Plakophilin 2 (P663) Polyclonal Antibody-BS3368

Plakophilin 2 (P663) Polyclonal Antibody

Catalogue Numbers: BS3368-50, BS3368-100

Sizes: 50µl, 100µl

Alternative Name: Plakophilin-2; PKP2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99959

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Plakophilin 2 (P663) polyclonal antibody detects endogenous levels of Plakophilin 2 protein.

Bioworld Molecular Weight: ~ 97 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Plakophilin 2 (P663) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate(20ug)
Lane 2: HCT116 whole cell lysate(20ug)
Lane 3: The Heart tissue lysate of Rat(20ug)

Immunogen: Synthetic peptide, corresponding to amino acids 634-680 of Human Plakophilin 2.

Conjugate: Unconjugated

Modification: Unmodified