PLK1 (Phospho-T210) Polyclonal Antibody-BS94045

PLK1 (Phospho-T210) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94045-50, BS94045-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P53350(Human)

Host: Rabbit

Reactivity: Human

Applications: WB ICC/IF FC

All Applications: WB:1:500-1:1,000
FC:1:10-1:50
ICC:1:10-1:50

Background: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. The phosphorylation of mouse, rat and human Plk on Thr 210 enhances Plk catalytic activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Flow cytometric analysis of MCF-7 cells with Phospho-PLK1 (T210) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as theSecondary antibody.

Bioworld Molecular Weight: 68 kDa

Specificity: PLK1 (Phospho-T210) polyclonal antibody detects endogenous levels of PLK1 protein only when phosphorylated at T210.

Alternative Name: PLK-1, STPK13, PLK1, Cell cycle regulated protein kinase, PLK 1, PLK, PLK-1, plk1, PLK1, Polo like kinase 1, Polo-like kinase 1, Serine/threonine protein kinase 13, Serine/threonine protein kinase PLK1, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase PLK1, STPK 13, STPK13,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr210 of human PLK1.

Conjugate: Unconjugated

Modification: Phosphorylation