Product Description

PMCA3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65711-50, BS65711-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P20020

Host: Rabbit

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Plasma membrane-type Ca2+-ATPases (PMCAs) mediate the export of bivalent calcium ions from eukaryotic cells. As members of the P class of ion-motive ATPases, PMCAs are a functionally diverse group of proteins that are derived from alternatively spliced transcripts originating from four distinct genes, PMCA1, 2, 3, and 4. The expression of different PMCA isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, and with respect to the physiological needs of specific cell and tissue types. Spatial and temporal rates of resting intracellular Ca2+ concentrations and Ca2+ signaling in eukaryotic cells are dependent on the array of PMCA isoforms that are expressed in concert with the rate of Ca2+ export. PMCA3 expression is confined to brain and skeletal muscle. The PMCA4 gene is located on human chromosome 1q25 and is ubiquitously expressed.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PMCA3 Polyclonal Antibody detects endogenous levels of PMCA3 protein.

Bioworld Molecular Weight: 134kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PMCA3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PMCA3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PMCA3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: ATP2B3; ATP2B3 protein; Calcium Pump PMCA3 ATPase; ATPase Ca++ transporting plasma membrane 3; Plasma membrane calcium ATPase 3; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium pump isoform 3; Plasma membrane calcium transporting ATPase 3; PMCA 3; PMCA3; PMCA3a; AT2B3_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human PMCA3/ATP2B1:351-450/1220 

Conjugate: Unconjugated

Modification: Unmodified