Product Description

PML (P39) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1296-50, BS1296-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15, 17) (q22, q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and resμlts in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, whichμltimately facilitates leukemogenesis.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Protein PML, Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19, MYL, PP8675, RNF71, TRIM19

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human PML.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PML (P39) polyclonal antibody detects endogenous levels of PML protein.

Molecular Weight: ~ 98 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P29590

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only