Product Description

PMS1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75572-50, BS75572-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P54277

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using PMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 110kDa

Alternative Name: PMS1; HNPCC3; MLH2; PMSL1; hPMS1

Immunogen: Recombinant fusion protein of human PMS1(NP_000525.1).

Conjugate: Unconjugated

Modification: Unmodified