Product Description

PNPT1 Polyclonal Antibody

Catalogue Numbers: BS62427-50, BS62427-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q8TCS8

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Mitochondrial polyribonucleotide nucleotidyltransferase, also designated 3'-5' RNA exonuclease, OLD35, PNPase or PNPT1, is an evolutionaly conserved protein in which the mouse protein shares 90% identity with the human version. PNPase participates in mRNA degradation and hydrolyzes single-stranded ribonucleotides in the 3' to 5' direction. PNPase forms homotrimers and is upregulated in response to interferon-β induction. The N-terminus of PNPase contains a putative mitochondrial targeting sequence; mutation analysis confirms that N-terminal sequences of PNPase target the protein to the mitochondria. Endogenous PNPase also co-localizes with a mitochondrial marker protein in HeLa cells.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -24°C long term. Avoid freeze-thaw cycles.

Specificity: PNPT1 polyclonal antibody detects endogenous levels of PNPT1 protein.

Extra Notes: Western blot (WB) analysis of PNPT1 polyclonal antibody at 1:500 dilution
LaneA:PC12 whole cell lysate
LaneB:MEF whole cell lysate
LaneC:A549 whole cell lysate
LaneD:L02 whole cell lysate
LaneE:HCT116 whole cell lysate

Bioworld Molecular Weight: ~ 88 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Polyribonucleotide nucleotidyltransferase 1, mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein; PNPT1; PNPASE

Immunogen: Synthetic peptide, corresponding to Human PNPT1.

Conjugate: Unconjugated

Modification: Unmodified