Product Description

Podocin Polyclonal Antibody

Catalogue Numbers: BS60405-50, BS60405-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NP85

Host: Rabbit

Reactivity: Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Podocin polyclonal antibody detects endogenous levels of Podocin protein.

Extra Notes: Western blot (WB) analysis of Podocin pAb at 1:1000 dilution
Lane1:The Kidney tissue lysate of Mouse(20ug)
Lane2:The Kidney tissue lysate of Rat(30ug)

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NPHS2

Immunogen: A synthetic peptide corresponding to residues in Human Podocin.

Conjugate: Unconjugated

Modification: Unmodified