POLG2 Polyclonal Antibody
Catalogue Numbers: BS61176-50, BS61176-100
Sizes: 50µl, 100µl
Alternative Name: DNA polymerase subunit gamma-2; mitochondrial; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta; POLG2
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UHN1
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol γ2 (polymerase (DNA directed), gamma 2, accessory subunit), also known as mitochondrial DNA polymerase accessory subunit, POLB, HP55, PEOA4, POLGB, MTPOLB, POLG-BETA or POLG2, is a 485 amino acid subunit of mitochondrial DNA pol γ. DNA pol γ2 enhances DNA binding and promotes processive DNA synthesis. Defects in the gene encoding DNA pol γ2 are the cause of PEOA4 (progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4).
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: POLG2 polyclonal antibody detects endogenous levels of POLG2 protein.
Bioworld Molecular Weight: ~ 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of POLG2 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: H9C2 whole cell lysate
Lane 4: SP20 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human POLG2.
Conjugate: Unconjugated
Modification: Unmodified