Product Description

POLI Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61707-50, BS61707-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA pol ι (pol iota), which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol ι is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol ι also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol ι acts sequentially with DNA Pol ζ, which is essential for damageinduced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol ι is likely to be highly mutagenic.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 630-680 of Human POLI.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: POLI polyclonal antibody detects endogenous levels of POLI protein.

Molecular Weight: ~ 83 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UNA4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only