Product Description

POLR1C Polyclonal Antibody

Catalogue Numbers: BS5916-50, BS5916-100

Sizes: 50µl, 100µl

Alternative Name: DNA-directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III; subunit AC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40; POLR1C; POLR1E

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15160

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RNA polymerases transcribe nuclear genes for ribosomal RNA, thus representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I transcription. RPA16, RPA40 and RPA135 are subunits of Pol I that associate with each other at an early stage of RNA Pol I assembly. RPA40 is essential for the function and integrity of the complex and is also an essential subunit of RNA polymerase III (Pol III).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLR1C polyclonal antibody detects endogenous levels of POLR1C protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of POLR1C polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: NIH-3T3 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human POLR1C.

Conjugate: Unconjugated

Modification: Unmodified