POLR2A (Phospho-S2) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94011-50, BS94011-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P24928(Human) P08775(Mouse) EntrezGene:363633 (Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IP, ICC/IF IHC FC
All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: POLR2A (Phospho-S2) polyclonal antibody detects endogenous levels of POLR2A protein only when phosphorylated at S2.
Bioworld Molecular Weight: 250 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti- Phospho-POLR2A (S2) antibody at 1/500 dilution.
Positive control:
Line 1: Hela
Line 2: MCF-7, ICC staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: RNA polymerase II subunit B1, POLR2A, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, Polymerase (RNA) II (DNA directed) polypeptide A, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1, RPBh1, RpIILS, RPO2, RPOL2,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser2 of human POLR2A.
Conjugate: Unconjugated
Modification: Phosphorylation