POLR2D Polyclonal Antibody-BS7304

POLR2D Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7304-50, BS7304-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15514

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100 | IP,1:50 - 1:200

Background: This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using POLR2D antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of A549 cells using POLR2D antibody., Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody. Western blot was performed from the immunoprecipitate using POLR2D antibody at a dilution of 1:1000.

Alternative Name: POLR2D; HSRBP4; HSRPB4; RBP4; RPB16

Immunogen: Recombinant fusion protein of human POLR2D(NP_004796.1).

Conjugate: Unconjugated

Modification: Unmodified