Product Description

POLR2E (L58) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3113-50, BS3113-100

Sizes: 50µl, 100µl

Swiss-Prot: P19388

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: POLR2E (L58) polyclonal antibody detects endogenous levels of POLR2E protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of POLR2E (L58) polyclonal antibody at 1:500 dilution
Lane 1: CT26 whole cell lysate (40ug)
Lane 2: PC12 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: HCT116 whole cell lysate (40ug)
Lane 5: 786-O whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of POLR2E (L58) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 25 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4; POLR2E

Immunogen: Synthetic peptide, corresponding to amino acids 30-80 of Human POLR2E.

Conjugate: Unconjugated

Modification: Unmodified