POLR2F Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75219-50, BS75219-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P61218
Host: Rabbit
Reactivity: Mouse
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of mouse pancreas, using POLR2F antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 17kDa
Alternative Name: POLR2F; HRBP14.4; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15
Immunogen: Recombinant fusion protein of human POLR2F(NP_068809.1).
Conjugate: Unconjugated
Modification: Unmodified