Product Description

POLR2I Polyclonal Antibody

Catalogue Numbers: BS60145-50, BS60145-100

Sizes: 50µl, 100µl

Alternative Name: DNA-directed RNA polymerase II subunit RPB9; RNA polymerase II subunit B9; DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RPB14.5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P36954

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. POLR2I (polymerase (RNA) II (DNA directed) polypeptide I), also known as RPB9 or hRPB14.5, is a 125 amino acid nuclear protein belonging to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. Component of RNA polymerase II, POLR2I catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2I is part of the upper jaw surrounding the central large cleft and is thought to grab the incoming DNA template.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLR2I polyclonal antibody detects endogenous levels of POLR2I protein.

Bioworld Molecular Weight: ~ 14 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of POLR2I pAb at 1:500 dilution
Lane 1: C6 whole cell lysate(40ug)
Lane 2: CT26 whole cell lysate(30ug)
Lane 3: HEK293T whole cell lysate(40ug)
Lane 4: L02 whole cell lysate(40ug)

Immunogen: A synthetic peptide corresponding to residues in Human POLR2I.

Conjugate: Unconjugated

Modification: Unmodified