POLR3A (P68) Polyclonal Antibody-BS3292

POLR3A (P68) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3292-50, BS3292-100

Sizes: 50µl, 100µl

Swiss-Prot: O14802

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: POLR3A (P68) polyclonal antibody detects endogenous levels of POLR3A protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of POLR3A (P68) pAb at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: K562 whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of POLR3A (P68) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 156 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; POLR3A

Immunogen: Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.

Conjugate: Unconjugated

Modification: Unmodified