Product Description

POLR3E Polyclonal Antibody

Catalogue Numbers: BS62354-50, BS62354-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NVU0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. POLR3E, also known as DNA-directed RNA polymerase III subunit RPC5, is a 708 amino acid nuclear protein that is one of the 17 subunits that comprise Pol III. Specifically, POLR3E is a DNA-dependent RNA polymerase that uses the four ribonucleoside triphosphates to catalyze the transcription of DNA to RNA. The gene encoding POLR3K maps to human chromosome 16, which encodes over 900 genes in approximately 90 million base pairs and is associated with a variety of genetic disorders.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLR3E polyclonal antibody detects endogenous levels of POLR3E protein.

Extra Notes: Western blot (WB) analysis of POLR3E polyclonal antibody at 1:500 dilution
LaneA:K562 whole cell lysate
LaneB:HEK293T whole cell lysate
LaneC:AML-12 whole cell lysate
LaneD:H9C2 whole cell lysate

Bioworld Molecular Weight: ~ 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide; POLR3E; KIAA1452

Immunogen: Synthetic peptide, corresponding to Human POLR3E.

Conjugate: Unconjugated

Modification: Unmodified