POU5F1 / OCT4 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB0046-50, MB0046-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P20263
Host: Mouse
Reactivity: Mouse
Applications: WB
All Applications: WB: 1:1000
Background: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotenc
Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins
Bioworld Molecular Weight: Predicted band size:45KDa
Observed band size:45KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western BlotDetection of POU5F1 antibody in 20ug and 40ug mESC cell lysates using POU5F1 antibody (1:1000 diluted).
Alternative Name: POU domain, class 5, transcription factor 1; NF-A3; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; Pou5f1; Oct-3; Oct-4; Otf-3; Otf3
Immunogen: Recombinant full length Human POU5F1.
Conjugate: Unconjugated
Modification: Unmodified