Product Description

POU6F1 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS8822-50, BS8822-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14863

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100

Background: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 45kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s., Immunofluorescence analysis of A-549 cells using POU6F1 antibody.

Alternative Name: POU6F1; BRN5; MPOU; TCFB1

Immunogen: Recombinant fusion protein of human POU6F1(NP_002693.3).

Conjugate: Unconjugated

Modification: Unmodified