PP2A alpha/beta Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11443-50, MB11443-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P67775
Host: Rabbit
Reactivity: Human, Mouse, Rat, Hamster
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 36 kDa; Observed MW: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PP2A alpha/beta in C2C12, 3T3, mouse brain lysates using PP2A alpha/beta antibody., Western blot analysis of PP2A alpha/beta in rat Brain, C6, CHO-K1, Hela lysates using PP2A alpha/beta antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PP2A alpha/beta antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of PP2A alpha/beta in hela using PP2A alpha/beta antibody, and DAPI.
Alternative Name: PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C
Immunogen: A synthetic peptide of human PP2A alpha
Conjugate: Unconjugated
Modification: Unmodified