PP2C-alpha Monoclonal Antibody
Catalogue Numbers: MB66469-50, MB66469-100
Sizes: 50µl, 100µl
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P35813
Host: Mouse
Reactivity: Human, Monkey
Applications: WB, FC
All Applications: WB (1/500 - 1/1000), FC (1/100 - 1/200)
Background: The α isoform of protein phosphatase 2C (PP2C-α) is the catalytic subunit of a widely expressed serine/threonine phosphatase involved in regulation of the cell stress response. Also known as magnesium-dependent protein phosphatase (PPM1A), this monomeric phosphatase is a member of a conserved group of proteins that acts on many different substrates in numerous pathways. PP2C-α inhibits p38 MAPK and SAPK/JNK pathways activated in response to cell stress as seen in both in vivo and in vitro studies. Specifically, PP2C-α removes phosphates from MKK3 and MKK7, reducing activity of both proteins and inhibiting activation of the downstream kinases JNK and p38 MAPK, respectively. Another PP2C-α substrate is IKKβ, the critical regulator of NF-κB signaling. Dephosphorylation of IKKβ at Ser177/181 by PPM1A and PPM1B results in inactivation of IKKβ and inhibition of NF-κB signaling. PP2C-α is one of the phosphatases responsible for removing phosphate residues from cyclin dependent protein kinases. In a study using HeLa cell extracts, PP2C-α dephospohrylates CDK2 and CDK6, with a preference toward interacting with CDK2 phosphorylated at Thr160, a residue found in the activating T-loop of the kinase. Removal of phosphates from this site is thought to inactivate cyclin-associated kinases. PP2C-α induces cell cycle arrest and apoptosis, likely through activation of p53 though other pathways may also contribute to PP2C-α mediated cell death . Additional PP2C-α substrates include the Wnt signaling pathway protein axin and CFTR, a chloride channel protein implicated in cystic fibrosis.
Purification and Purity: The antibody was purified by protein G.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of PP2C-alpha protein.
Extra Notes: Western blot analysis of PP2C-alpha expression in COS7 (A), Jurkat (B), A431 (C), HeLa (D), HEK293 (E), Raji (F), MCF7 (G) whole cell lysates.
Bioworld Molecular Weight: ~ 43 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
Immunogen: Recombinant fragment of human PP2C-alpha expressed in E. Coli
Conjugate: Unconjugated
Modification: Unmodified