Product Description

PP2C-alpha Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66469-50, MB66469-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The α isoform of protein phosphatase 2C (PP2C-α) is the catalytic subunit of a widely expressed serine/threonine phosphatase involved in regμlation of the cell stress response. Also known as magnesium-dependent protein phosphatase (PPM1A), this monomeric phosphatase is a member of a conserved group of proteins that acts on many different substrates in numerous pathways. PP2C-α inhibits p38 MAPK and SAPK/JNK pathways activated in response to cell stress as seen in both in vivo and in vitro studies. Specifically, PP2C-α removes phosphates from MKK3 and MKK7, reducing activity of both proteins and inhibiting activation of the downstream kinases JNK and p38 MAPK, respectively. Another PP2C-α substrate is IKKβ, the critical regμlator of NF-κB signaling. Dephosphorylation of IKKβ at Ser177/181 by PPM1A and PPM1B resμlts in inactivation of IKKβ and inhibition of NF-κB signaling. PP2C-α is one of the phosphatases responsible for removing phosphate residues from cyclin dependent protein kinases. In a study using HeLa cell extracts, PP2C-α dephospohrylates CDK2 and CDK6, with a preference toward interacting with CDK2 phosphorylated at Thr160, a residue found in the activating T-loop of the kinase. Removal of phosphates from this site is thought to inactivate cyclin-associated kinases. PP2C-α induces cell cycle arrest and apoptosis, likely through activation of p53 though other pathways may also contribute to PP2C-α mediated cell death . Additional PP2C-α substrates include the Wnt signaling pathway protein axin and CFTR, a chloride channel protein implicated in cystic fibrosis.

Category: Primary Antibody

Reactivity: Human, Monkey

Host: Mouse

Applications: WB, FC

Alternate Names: PPPM1A, Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fragment of human PP2C-alpha expressed in E. Coli

Dilution: WB (1/500 - 1/1000), FC (1/100 - 1/200)

Purification: The antibody was purified by protein G.

Specificity: Recognizes endogenous levels of PP2C-alpha protein.

Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P35813

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only