Product Description

PPARβ Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Catalogue Numbers: GCP114-50, GCP114-100

Sizes: 50µl, 100µl

Swiss-Prot: Q03181

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis . In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes. PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8). PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs . More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity. Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PPARβ polyclonal antibody detects endogenous levels of PPARβ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PPARβ polyclonal antibody at 1:1000 dilution
Lane 1: EC9706 whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Rat Brain using PPARβ antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 51 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Peroxisome proliferator-activated receptor delta; PPAR-delta; NUCI; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta; PPARD; NR1C2; PPARB

Immunogen: Synthetic peptide, corresponding to Human PPARβ.

Conjugate: Unconjugated

Modification: Unmodified