Product Description

PPP2R1B Polyclonal Antibody

Catalogue Numbers: BS5862-50, BS5862-100

Sizes: 50µl, 100µl

Alternative Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A-Aβ; PP2AAβ; PP2A Aβ; PP2A-APR65β; PP2A -AR1β;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30154

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-Aα and -Aβ, PP2A-Bα and -Bβ, PP2A-Cα and -Cβ, and PP2A-B56α and -B56β.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PPP2R1B polyclonal antibody detects endogenous levels of PPP2R1B protein.

Bioworld Molecular Weight: ~ 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PP2A-Aβ (F590) pAb at 1:1000 dilution
Lane 1: A2780 whole cell lysate(40ug)
Lane 2: Beas-2B whole cell lysate(40ug)
Lane 3: Myla2059 whole cell lysate(40ug)
Lane 4: 3T3-L1 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 556-601 of Human PPP2R1B.

Conjugate: Unconjugated

Modification: Unmodified