Product Description

PPP4R2 Polyclonal Antibody

Catalogue Numbers: BS62475-50, BS62475-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q9NY27

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Serine/threonine-protein phosphatase 4 regulatory subunit 2, regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Specificity: PPP4R2 polyclonal antibody detects endogenous levels of PPP4R2 protein.

Extra Notes: Western blot (WB) analysis of PPP4R2 polyclonal antibody at 1:500 dilution
LaneA:CT-26 whole cell lysate
LaneB:PC12 whole cell lysate
LaneC:Hela whole cell lysate

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Serine/threonine-protein phosphatase 4 regulatory subunit 2; PPP4R2; SBBI57

Immunogen: Synthetic peptide, corresponding to Human PPP4R2.

Conjugate: Unconjugated

Modification: Unmodified