PR Polyclonal Antibody-GCP29

PR Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Catalogue Numbers: GCP29-50, GCP29-100

Sizes: 50µl, 100µl

Swiss-Prot: P06401

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PR polyclonal antibody detects endogenous levels of PR protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PR polyclonal antibody at 1:2000 dilution
Lane 1: MCF-7 whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Human Brain using PR antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 125 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

Immunogen: Synthetic peptide, corresponding to Human PR.

Conjugate: Unconjugated

Modification: Unmodified