Product Description

PRDM12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67212-50, BS67212-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9H4Q4

Host: Rabbit

Reactivity: Human

Applications: WB, IF/IC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Background: Involved in the positive regulation of histone H3-K9 dimethylation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 173% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of PRDM12 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PRDM12 expression in U87MG (A), MCF7 (B) whole cell lysates., Immunofluorescent analysis of PRDM12 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: PFM9; PR domain zinc finger protein 12; PR domain-containing protein 12

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRDM12. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified