Product Description

PRG2 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67313-50, BS67313-100

Sizes: 50µl, 100µl

Swiss-Prot: P13727

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: The eosinophil major basic protein (EMBP), also designated MBP, PRG2, proteoglycan 2, BMPG, or bone marrow natural killer cell activator, is a constituent of the crystalline core of the eosinophil granule. High levels of the pro-EMBP are present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A
(PAPPA), angiotensinogen (AGT) and C3δγ. EMBP may influence antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and may play a role in immune hypersensitivity reactions. EMBP stimulates an Src kinase-dependent activation of class I (A) phosphoinositide 3-kinase and, in turn, activation of protein kinase C ζ in neutrophils. EMBP transcription is under regulation by novel combinatorial interactions of GATA-1, PU.1, and C/EBPε isoforms.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of PRG2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PRG2 expression in MCF7 (A), HL60 (B), mouse kidney (C), mouse heart (D) whole cell lysates., Immunohistochemical analysis of PRG2 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 25 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: MBP; Bone marrow proteoglycan; BMPG; Proteoglycan 2

Immunogen: Recombinant full length protein of human PRG2

Conjugate: Unconjugated

Modification: Unmodified