Product Description

PRGC1 (T56) Polyclonal Antibody

Catalogue Numbers: AP0789-50, AP0789-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q9UBK2

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: PPARγ coactivator-1α (PGC-1α) was originally identified as a transcriptional coactivator whose expression closely correlated with adaptive thermogenesis following exposure to cold temperatures. Named for its association with the nuclear receptor peroxisome-proliferator activated receptor (PPARγ), PGC-1α interacts with a diverse array of transcription factors to regulate numerous aspects of cell physiology. PGC-1α helps to regulate cell processes important in adaptive thermogenesis and energy metabolism, including the related functions of glucose uptake, gluconeogenesis, insulin secretion, and mitochondrial biogenesis. Long thought to be a potential therapeutic target for the treatment of type II diabetes, obesity, cardiomyopathy, or other metabolic disorders (reviewed in 4), a recent functional survey found no obvious differences in PPARγ activity associated with recognized PGC-1α variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PRGC1 (T56) polyclonal antibody detects endogenous levels of PRGC1 protein.

Extra Notes: Western blot (WB) analysis of PRGC1 (T56) polyclonal antibody at 1:1000 dilution
Lane1:PC12 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:THP-1 whole cell lysate(40ug)
Lane4:Hela whole cell lysate(40ug)
Lane5:HepG2 whole cell lysate(20ug)

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6; PPARGC1A; LEM6; PGC1; PGC1A; PPARGC1

Immunogen: Synthetic peptide, corresponding to Human PRGC1.

Conjugate: Unconjugated

Modification: Unmodified