PRIM1 (K413) Polyclonal Antibody-BS3851

PRIM1 (K413) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3851-50, BS3851-100

Sizes: 50µl, 100µl

Swiss-Prot: P49642

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PRIM1 (K413) polyclonal antibody detects endogenous levels of PRIM1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRIM1 (K413) pAb at 1:1000 dilution
Lane 1: HepG2 whole cell lysate (20ug)
Lane 2: K562 whole cell lysate (20ug)
Lane 3: HEK293T whole cell lysate (20ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of PRIM1 (K413) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA primase small subunit; DNA primase 49 kDa subunit; p49; PRIM1

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human PRIM1.

Conjugate: Unconjugated

Modification: Unmodified