Product Description

PRKAR1A (PKA regμlatory subunit I alpha) Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4153-25, MB4153-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: cAMP is a signaling molecμle important for a variety of cellμlar functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regμlatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regμlatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regμlatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regμlatory subunits. This protein was found to be a tissue-specific extinguisher that down-regμlates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: IF, IHC, WB

Alternate Names: ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Fμll-length protein expressed in 293T cell transfected with human PRKAR1A expression vector

Dilution: WB 1:2000, IHC 1:50, IF 1:100

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG1

Molecular Weight: 43.0 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P10644

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only