PRL Polyclonal Antibody
Catalogue Numbers: BS60888-50, BS60888-100
Sizes: 50µl, 100µl
Alternative Name: Prolactin; PRL; PRL
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01236
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Protein tyrosine phosphatases (PTPs) play a role in regulating diverse cellular processes. They form a small class of prenylated protein phosphatases called PRL proteins characterized by a C-terminal consensus sequence for prenylation. PRL-1, also designated Protein tyrosine phosphatase type IVA protein 1 (PTP4A1), is a unique nuclear PTP that is induced in regenerating liver and mitogen-stimulated cells. It is primarily expressed in spleen, bone marrow, thymus, lymph nodes, T lymphocytes and tonsil and is overexpressed in tumor cell lines. PRL-2 (Protein tyrosine phosphatase type IVA protein 2, or PTP4A2) is ubiquitously expressed with highest levels in heart, skeletal muscle and thymus but is also overexpressed in prostate tumor tissue. PPRL-2 is stimulates progression from G1 into S phase during mitosis and promotes tumors. PRL-3, also known as Protein Tyrosine Phosphatase Type IVA, member 3 (PTP4A3) is expressed in heart and skeletal muscle as well as epithelial cells of the small intestine and associates with the cell plasma membrane. Over expression of PRL-3 inhibits angiotensin-II induced cell calcium mobilization and promotes cell growth. PRL-3 is important for colorectal cancer metastasis and may serve as a new therapeutic target for this condition.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PRL polyclonal antibody detects endogenous levels of PRL protein.
Bioworld Molecular Weight: ~ 26 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of PRL polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: H9C2 whole cell lysate
Lane 4: SP20 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human PRL.
Conjugate: Unconjugated
Modification: Unmodified