Product Description

PRMT5 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9940M-50, BS9940M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14744

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB: 1:1000-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200

Background: PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PRMT5 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PRMT5 Rabbit mAb at 1:1000 dilution
Lane 1: A431 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody.Counter stained with hematoxylin., ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs; PRMT5; HRMT1L5; IBP72; JBP1; SKB1

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified