pro Caspase 3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91108-50, BS91108-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P42574(Human) P70677(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: pro Caspase 3 polyclonal antibody detects endogenous levels of pro Caspase 3 protein.
Bioworld Molecular Weight: 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of pro Caspase 3 on Jurkat cells lysates using anti-pro Caspase 3 antibody at 1/1,000 dilution., ICC staining pro Caspase 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CASP-3, CPP-32, SCA-1, CASP3, CASP3, Caspase 3, Caspase 3 apoptosis related cysteine peptidase, CPP32, CPP32B, Procaspase 3, SCA 1, SCA1, Yama, Yama protein,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified