pro Caspase 7 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91109-50, BS91109-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P55210(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: A unique family of Cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: pro Caspase 7 polyclonal antibody detects endogenous levels of pro Caspase 7 protein.
Bioworld Molecular Weight: 34 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of pro Caspase 7 on human lung lysates using anti-pro Caspase 7 antibody at 1/1,000 dilution., ICC staining pro Caspase 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CASP-7, ICE-LAP3, CASP7, apoptosis-related cysteine peptidase, Apoptotic protease Mch-3, CASP-7, CASP7, CASP7, Caspase-7 subunit p11, CMH-1, ICE-LAP3, ICE-like apoptotic protease 3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified