Product Description

pro Caspase 9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91110-50, BS91110-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55211(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC FC

All Applications: WB:1:1,000-1:2,000
FC:1:10-1:50
IHC:1:50-1:200

Background: A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: pro Caspase 9 polyclonal antibody detects endogenous levels of pro Caspase 9 protein.

Bioworld Molecular Weight: 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of pro Caspase 9 on Hela cells lysates using anti-pro Caspase 9 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-pro Caspase 9 antibody. Counter stained with hematoxylin.

Alternative Name: CASP-9, APAF-3, ICE-LAP6, CASP9, APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9, Caspase-9 subunit p10, ICE-LAP6, ICE-like apoptotic protease 6, ICELAP6, MCH6,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified