Profilin 1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91111-50, BS91111-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07737(Human) P62962(Mouse) P62963(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Profilins regulate Actin polymerization by binding to and sequestering the Actin monomer. Profilins act as a nucleotide exchange factor that charges Actin with ATP after binding the Actin monomer through a 1:1 stoichiometric relationship. Human Profilin-1 and Profilin-2 are encoded by two separate genes mapping to chromosomes 17p13.2 and 3q25.1, respectively. Both Profilin-1 and Profilin-2 are abundantly expressed in kidney. Profilin-1 is highly expressed in lung, liver, placenta and kidney while Profilin-2 is highly expressed in brain and skeletal muscle. In axonal and dendritic processes of mouse brain, Profilins co-localize with dynamin I and synapsin. Profilin may play a role in mediating cell adhesion. The overexpression of Profilin in endothelial cells results in increased adhesion to Fibronectin. In food allergy, plant Profilin is considered a pan-allergen. Case studies indicate individuals with allergies to various foods including celery, carrots, zucchini and peanuts are actually sensitive to the Profilin proteins in these foods.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Profilin 1 polyclonal antibody detects endogenous levels of Profilin 1 protein.
Bioworld Molecular Weight: 15 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution., ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PFN1, Actin binding protein, ALS18, Epididymis tissue protein Li 184a, OTTHUMP00000125244, PFN 1, Pfn, PFN1, PROF1, Profilin I, Profilin-1, Profilin1, ProfilinI,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified