Product Description

Profilin-2 Polyclonal Antibody

Catalogue Numbers: BS60364-50, BS60364-100

Sizes: 50µl, 100µl

Alternative Name: Profilin2; Profilin 2; Profilin II; PFN2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35080

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Profilins (Profilin-1, Profilin-2 and Profilin-3) act as nucleotide exchange factors that use ATP to charge Actin, a process that regulates Actin polymerization by subsequently sequestering the Actin monomer. Profilin-2, also known as PFN2 or PFL, is a 140 amino acid protein that is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the Profilin family, Profilin-2 functions as an Actin monomer-binding protein that influences the structure of the cytoskeleton by regulating Actin polymerization in response to extracellular signals. High levels of Profilin-2 inhibit Actin polymerization, while lower levels enhance Actin polymerization, suggesting that the expression of Profilin-2 is regulated by the need for polymerization within the cell. Two isoforms of Profilin-2 exist due to alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Profilin-2 polyclonal antibody detects endogenous levels of Profilin-2 protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Profilin-2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat skeletal muscle tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human Profilin-2.

Conjugate: Unconjugated

Modification: Unmodified