Product Description

Prohibitin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12206-50, MB12206-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P35232

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: PHB1 is 30kDa tumor suppressor protein involved in cell cycle control. PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation. In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 30 kDa; Observed MW: 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of Prohibitin in Hela using Prohibitin antibody, and DAPI., Western blot analysis of Prohibitin in Hela, A549, HL-60, U2OS, C6 lysates using Prohibitin antibody., Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Prohibitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: PHB1; HEL-215; HEL-S-54e

Immunogen: A synthetic peptide of human Prohibitin

Conjugate: Unconjugated

Modification: Unmodified