Product Description

Prolyl hydroxylase PHD1 (EGLN2) Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS9885M-50, BS9885M-100

Swiss-Prot: Q96KS0

Host: Rabbit

Prolyl hydroxylase PHD1 (EGLN2) Polyclonal Antibody-BS9885M

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:20 - 1:50

Background: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 43kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug Prolyl hydroxylase PHD1 ) antibody. Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 ) antibody at a dilution of 1:1000.

Alternative Name: EGLN2; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1

Immunogen: A synthetic peptide of human hydroxylase PHD1 (EGLN2))(NP_444274.1).

Modification: Unmodified

Conjugate: Unconjugated