PRP19 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91118-50, BS91118-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UMS4(Human) Q99KP6(Mouse) Q9JMJ4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: PRP19 polyclonal antibody detects endogenous levels of PRP19 protein.
Bioworld Molecular Weight: 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution., ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: hPso4, PRPF19, hPso4, NMP200, Nuclear matrix protein 200, Nuclear matrix protein NMP200 related to splicing factor PRP19, pre mRNA processing factor 19, Pre-mRNA-processing factor 19, PRP19, PRP19/PSO4 homolog, PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae), PRP19, PRPF19, PSO4, psoralen 4, Senescence evasion factor, SNEV, UBOX4,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified